DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins.

ABSTRACT: The delivery of antigens as DNA vaccines is an efficient alternative to induce immune responses against antigens, which are difficult to produce in recombinant form. However, the delivery of naked DNA is ineffective or relies on sophisticated ballistic devices. Here, we show a combination of liposome application and naked DNA vaccine that successfully overcomes these problems. Upon entrapment of plasmids encoding different antigens in cationic particles, transfection efficiencies similar to commercial kits were achieved in in vitro cell cultures. The liposome-based approach provided strong humoral responses against three malarial antigens, namely the Circumsporozoite protein and the C terminus of merozoite surface protein 1 from Plasmodium vivax (titers 10⁴ or 10³

SUBMITTER: Fotoran WL 

PROVIDER: S-EPMC5581859 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature