Biology and Genomics of an Historic Therapeutic Escherichia coli Bacteriophage Collection.

ABSTRACT: We have performed microbiological and genomic characterization of an historic collection of nine bacteriophages, specifically infecting a K1 E. coli O18:K1:H7 ColV⁺ strain. These phages were isolated from sewage and tested for their efficacy in vivo for the treatment of systemic E. coli infection in a mouse infection model by Smith and Huggins (1982). The aim of the study was to identify common microbiological and genomic characteristics, which co-relate to the performance of these phages in in vivo study. These features will allow an informed selection of phages for use as therapeutic agents. Transmission electron microscopy showed that six of the nine phages were Podoviridae and the remaining three were Siphoviridae. The four best perf

SUBMITTER: Baig A 

PROVIDER: S-EPMC5582158 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature