Computational Identification of MicroRNAs and Their Transcript Target(s) in Field Mustard (Brassica rapa L.).

ABSTRACT:

Background

Micro RNAs (miRNAs) are a pivotal part of non-protein-coding endogenous small RNA molecules that regulate the genes involved in plant growth and development, and respond to biotic and abiotic environmental stresses posttranscriptionally.

Objective

In the present study, we report the results of a systemic search for identification of new miRNAs in B. rapa using homology-based ESTs (Expressed Sequence Tags) analysis and considering a series of fi ltration criteria.

Materials and methods

Plant mature miRNA sequences were searched in non-protein coding ESTs registered in NCBI EST database. Zuker RNA folding algorithm was used to generate the secondary structures of the ESTs. Potential sequences were candidate as miRNA genes and characterized evolutionari

SUBMITTER: Hajieghrari B 

PROVIDER: S-EPMC5582250 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature