Cloning, Overexpression and in vitro Antifungal Activity of Zea Mays PR10 Protein.

ABSTRACT:

Background

Plants have various defense mechanisms such as production of antimicrobial peptides, particularly pathogenesis related proteins (PR proteins). PR10 family is an essential member of this group, with antifungal, antibacterial and antiviral activities.

Objective

The goal of this study is to assess the antifungal activity of maize PR10 against some of fungal phytopathogens.

Materials and methods

Zea mays PR10 gene (TN-05-147) was cloned from genomic DNA and cDNA and overexpressed in Escherichia coli. The existence of a 77- bp intron and two exons in PR10 was confi rmed by comparing the genomic and cDNA sequences. The PR10 cDNA was cloned in pET26b (+) expression vector and transformed into E. coli strain Rosetta DE3 in order t

SUBMITTER: Zandvakili N 

PROVIDER: S-EPMC5582252 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature