Two functionally distinct NADP⁺-dependent ferredoxin oxidoreductases maintain the primary redox balance of Pyrococcus furiosus.

ABSTRACT: Electron bifurcation has recently gained acceptance as the third mechanism of energy conservation in which energy is conserved through the coupling of exergonic and endergonic reactions. A structure-based mechanism of bifurcation has been elucidated recently for the flavin-based enzyme NADH-dependent ferredoxin NADP⁺ oxidoreductase I (NfnI) from the hyperthermophillic archaeon Pyrococcus furiosus. NfnI is thought to be involved in maintaining the cellular redox balance, producing NADPH for biosynthesis by recycling the two other primary redox carriers, NADH and ferredoxin. The P. furiosus genome encodes an NfnI paralog termed NfnII, and the two are differentially expressed, depending on the growth conditions. In this study, we show that deletion of the genes encodi

SUBMITTER: Nguyen DMN 

PROVIDER: S-EPMC5582851 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature