Three distinct 3-methylcytidine (m³C) methyltransferases modify tRNA and mRNA in mice and humans.

ABSTRACT: Chemical RNA modifications are central features of epitranscriptomics, highlighted by the discovery of modified ribonucleosides in mRNA and exemplified by the critical roles of RNA modifications in normal physiology and disease. Despite a resurgent interest in these modifications, the biochemistry of 3-methylcytidine (m³C) formation in mammalian RNAs is still poorly understood. However, the recent discovery of trm141 as the second gene responsible for m³C presence in RNA in fission yeast raises the possibility that multiple enzymes are involved in m³C formation in mammals as well. Here, we report the discovery and characterization of three distinct m³C-contributing enzymes in mice and humans. We found that methyltransferase-like (METTL) 2

SUBMITTER: Xu L 

PROVIDER: S-EPMC5582859 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature