A new approach for generating bispecific antibodies based on a common light chain format and the stable architecture of human immunoglobulin G₁.

ABSTRACT: Bispecific antibodies combine two different antigen-binding sites in a single molecule, enabling more specific targeting, novel mechanisms of action, and higher clinical efficacies. Although they have the potential to outperform conventional monoclonal antibodies, many bispecific antibodies have issues regarding production, stability, and pharmacokinetic properties. Here, we describe a new approach for generating bispecific antibodies using a common light chain format and exploiting the stable architecture of human immunoglobulin G₁ We used iterative experimental validation and computational modeling to identify multiple Fc variant pairs that drive efficient heterodimerization of the antibody heavy chains. Accelerated stability studies enabled selection of one Fc variant pair du

SUBMITTER: De Nardis C 

PROVIDER: S-EPMC5582861 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature