The atypical genesis and bioavailability of the plant-based small RNA MIR2911: Bulking up while breaking down.

ABSTRACT:

Scope

The uptake of dietary plant small RNAs (sRNAs) in consumers remains controversial, which is mainly due to low dietary content in combination with poor fractional absorption. MIR2911, among all the plant sRNAs including microRNAs, has been shown to be one of the most robustly absorbed sRNAs. Here we analyze the unusual abundance and unique genesis of MIR2911 during vegetable processing.

Methods and results

Using qRT-PCR, the abundance of MIR2911 increased dramatically in macerated tissues while other microRNAs degraded. The accumulation of MIR2911 correlated with the degradation of the rRNAs, consistent with MIR2911 being derived from the 26S rRNA. Bioinformatic analysis predicts a microRNA-like precursor structure for MIR2911; however, no reciprocal increase in the put

SUBMITTER: Yang J 

PROVIDER: S-EPMC5583006 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature