Effects of cycloheximide on the interpretation of ribosome profiling experiments in Schizosaccharomyces pombe.

ABSTRACT: Stress conditions lead to global and gene-specific changes in RNA translation. Ribosome profiling experiments have identified genome-wide alterations in the distribution of ribosomes along mRNAs. However, it is contentious whether these changes reflect real responses, or whether they are artefacts caused by the use of inhibitors of translation (notably cycloheximide). To address this issue we performed ribosome profiling with the fission yeast Schizosaccharomyces pombe under conditions of exponential growth (unstressed) and nitrogen starvation (nutritional stress), and both in the presence and absence of cycloheximide. We examined several aspects of the translational response, including density of ribosomal footprints on coding sequences, 5' leader ribosomal densities, distribution of ribo

SUBMITTER: Duncan CDS 

PROVIDER: S-EPMC5583251 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature