Time-Resolved Pharmacological Studies using Automated, On-line Monitoring of Five Parallel Suspension Cultures.

ABSTRACT: Early stage pharmacological studies rely on in vitro methodologies for screening and testing compounds. Conventional assays based on endpoint measurements provide limited information because the lack in temporal resolution may not determine the pharmacological effect at its maximum. We developed an on-line, automated system for near real-time monitoring of extracellular content from five parallel suspension cultures, combining cell density measurements with a high-resolution separations every 12 minutes for 4 days. Selector and switching valves provide the fluidic control required to sample from one culture during the analysis of the previous sample from another culture, a time-saving measure that is fundamental to the throughput of the presented system. The system was applied to study the

SUBMITTER: Alhusban AA 

PROVIDER: S-EPMC5583285 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature