A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.

ABSTRACT: Conventional genetic screens for recessive mutants are inadequate for studying biological processes in the adult vertebrate due to embryonic lethality. Here, we report that a novel inducible mutagenesis system enables to study gene function in both embryonic and adult zebrafish. This system yields genetic mutants with conditional ectopic over- or under-expression of genes in F₁ heterozygotes by utilizing inducible Tet-On transcriptional activation of sense or anti-sense transcripts from entrapped genes by Tol2 transposase-meditated transgenesis. Pilot screens identified 37 phenotypic mutants displaying embryonic defects (34 lines), adult fin regeneration defects (7 lines), or defects at both stages (4 lines). Combination of various techniques (such as: generating a new mutant al

SUBMITTER: Ma Z 

PROVIDER: S-EPMC5583359 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature