Modification of Sialic Acids on Solid Phase: Accurate Characterization of Protein Sialylation.

ABSTRACT: Sialic acids play many important roles in several physiological and pathological processes, including cancers, infection, and blood diseases. Sialic acids are fragile and prone to fragmentation under electrospray ionization and matrix-assisted laser desorption/ionization. It is crucial to modify sialic acids for qualitative and quantitative identification of their change in abundance in complex biological samples. Permethylation is a method of choice for sialic acid stabilization, but the harsh conditions during permethylation may lead to the decomposition of O-acetyl groups. Esterification or amidation in solution effectively protects sialic acids, yet it is not trivial to purify glycans from their reagents. Quantitative analysis of glycans can be achieved by labeling their reducing end u

SUBMITTER: Yang S 

PROVIDER: S-EPMC5583724 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature