Regulation of Noncoding Transcriptome in Developing Photoreceptors by Rod Differentiation Factor NRL.

ABSTRACT:

Purpose

Transcriptome analysis by next generation sequencing allows qualitative and quantitative profiling of expression patterns associated with development and disease. However, most transcribed sequences do not encode proteins, and little is known about the functional relevance of noncoding (nc) transcriptome in neuronal subtypes. The goal of this study was to perform a comprehensive analysis of long noncoding (lncRNAs) and antisense (asRNAs) RNAs expressed in mouse retinal photoreceptors.

Methods

Transcriptomic profiles were generated at six developmental time points from flow-sorted Nrlp-GFP (rods) and Nrlp-GFP;Nrl-/- (S-cone like) mouse photoreceptors. Bioinformatic analysis was performed to identify novel noncoding transcripts and assess their regulation by rod differ

SUBMITTER: Zelinger L 

PROVIDER: S-EPMC5584472 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature