The molecular speciation of soil-transmitted helminth eggs collected from school children across six endemic countries.

ABSTRACT:

Background

The diagnosis of soil-transmitted helminths (STHs; Ascaris, Trichuris and hookworms) is traditionally based on the demonstration of eggs in stool using microscopic techniques. While molecular techniques are more appropriate to speciate STH species they are seldom applied. In this study we speciated STH eggs from stool using molecular techniques to gain insights into the distribution of both human and animal STH species in the human host.

Methods

We speciated 207 STH egg isolates from stool collected during the baseline survey of six drug efficacy trials conducted in Brazil, Cambodia, Cameroon, Ethiopia, Tanzania and Vietnam applying a PCR - restriction fragment length polymorphisms based approach.

Results

DNA of Ascaris was detected in 71 (34.3%) samples, o

SUBMITTER: George S 

PROVIDER: S-EPMC5584671 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature