Genetic interaction mapping in mammalian cells using CRISPR interference.

ABSTRACT: We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein-protein-interaction data revealed a functional map of chromatin regulation.

SUBMITTER: Du D 

PROVIDER: S-EPMC5584685 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature