Barcoded NS31/AML2 primers for sequencing of arbuscular mycorrhizal communities in environmental samples.

ABSTRACT:

Premise of the study

Arbuscular mycorrhizal fungi (AMF) are globally important root symbioses that enhance plant growth and nutrition and influence ecosystem structure and function. To better characterize levels of AMF diversity relevant to ecosystem function, deeper sequencing depth in environmental samples is needed. In this study, Illumina barcoded primers and a bioinformatics pipeline were developed and applied to study AMF diversity and community structure in environmental samples.

Methods

Libraries of small subunit ribosomal RNA fragment amplicons were amplified from environmental DNA using a single-step PCR reaction with barcoded NS31/AML2 primers. Amplicons were sequenced on an Illumina MiSeq sequencer using version 2, 2 × 250-bp paired-end chemistry, and analyzed us

SUBMITTER: Morgan BST 

PROVIDER: S-EPMC5584815 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature