Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators.

ABSTRACT: Monitoring voltage dynamics in defined neurons deep in the brain is critical for unraveling the function of neuronal circuits but is challenging due to the limited performance of existing tools. In particular, while genetically encoded voltage indicators have shown promise for optical detection of voltage transients, many indicators exhibit low sensitivity when imaged under two-photon illumination. Previous studies thus fell short of visualizing voltage dynamics in individual neurons in single trials. Here, we report ASAP2s, a novel voltage indicator with improved sensitivity. By imaging ASAP2s using random-access multi-photon microscopy, we demonstrate robust single-trial detection of action potentials in organotypic slice cultures. We also show that ASAP2s enables two-photon imaging of g

SUBMITTER: Chamberland S 

PROVIDER: S-EPMC5584994 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature