Mitochondrial permeability transition pore: sensitivity to opening and mechanistic dependence on substrate availability.

ABSTRACT: Mitochondrial Ca²⁺ uptake has a key role in cellular Ca²⁺ homeostasis. Excessive matrix Ca²⁺ concentrations, especially when coincident with oxidative stress, precipitate opening of an inner mitochondrial membrane, high-conductance channel: the mitochondrial permeability transition pore (mPTP). mPTP opening has been implicated as a final cell death pathway in numerous diseases and therefore understanding conditions dictating mPTP opening is crucial for developing targeted therapies. Here, we have investigated the impact of mitochondrial metabolic state on the probability and consequences of mPTP opening. Isolated mitochondria were energised using NADH- or FADH₂-linked substrates. The functional consequences of Ca²⁺-induced mPTP opening

SUBMITTER: Briston T 

PROVIDER: S-EPMC5585167 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature