β Subunits Control the Effects of Human Kv4.3 Potassium Channel Phosphorylation.

ABSTRACT: The transient outward K⁺ current, I_to, activates early in the cardiac myocyte action potential, to begin repolarization. Human I_to is generated primarily by two Kv4.3 potassium channel α subunit splice variants (Kv4.3L and Kv4.3S) that diverge only by a C-terminal, membrane-proximal, 19-residue stretch unique to Kv4.3L. Protein kinase C (PKC) phosphorylation of threonine 504 within the Kv4.3L-specific 19-residues mediates α-adrenergic inhibition of I_to in human heart. Kv4.3 is regulated in human heart by various β subunits, including cytosolic KChIP2b and transmembrane KCNEs, yet their impact on the functional effects of human Kv4.3 phosphorylation has not been reported. Here, this gap in knowledge was addressed using human K

SUBMITTER: Abbott GW 

PROVIDER: S-EPMC5585193 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature