Image-based closed-loop feedback for highly mono-dispersed microdroplet production.

ABSTRACT: Micron-scale droplets isolated by an immiscible liquid can provide miniaturised reaction vessels which can be manipulated in microfluidic networks, and has seen a rapid growth in development. In many experiments, the precise volume of these microdroplets is a critical parameter which can be influenced by many external factors. In this work, we demonstrate the combination of imaging-based feedback and pressure driven pumping to accurately control the size of microdroplets produced in a microfluidic device. The use of fast-response, pressure-driving pumps allows the microfluidic flow to be quickly and accurately changed, while directly measuring the droplet size allows the user to define the more meaningful parameters of droplet size and generation frequency rather than flow rates or pressur

SUBMITTER: Crawford DF 

PROVIDER: S-EPMC5585215 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature