Structural and pharmacological basis for the induction of mitochondrial biogenesis by formoterol but not clenbuterol.

ABSTRACT: Mitochondrial dysfunction is associated with numerous acute and chronic degenerative diseases. The beta-2 adrenergic receptor (β₂AR) agonist formoterol induces mitochondrial biogenesis (MB), but other β₂AR agonists, such as clenbuterol, do not. We sought to identify the MB signaling pathway of formoterol and the differences in signaling between these two ligands that result in the differential induction of MB. While formoterol and clenbuterol increased cAMP, only formoterol increased the phosphorylation of Akt and its downstream target eNOS. The increase in Akt phosphorylation was Gβγ- and PI3K-dependent, and the increase in eNOS phosphorylation was Gβγ- and Akt-dependent. Only formoterol increased cGMP. Formoterol induced MB as measured by increases in uncoupled cell

SUBMITTER: Cameron RB 

PROVIDER: S-EPMC5585315 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature