The Accelerated Evolution of Lagging Strand Genes Is Independent of Sequence Context.

ABSTRACT: We previously discovered that lagging strand genes evolve faster in Bacillus subtilis (and potentially other bacteria). Lagging strand genes are transcribed in the head-on orientation with respect to DNA replication, leading to collisions between the two machineries that stall replication and can destabilize genomes. Our previous work indicated that the increased mutagenesis of head-on genes depends on transcription-coupled repair and the activity of an error prone polymerase which is likely activated in response to these collisions. Recently, it was proposed that sequence context is a major contributor to the increased mutagenesis and evolution of head-on genes. These models are based on laboratory-based evolution experiments performed in B. subtilis. However, critical evolutionary analys

SUBMITTER: Merrikh CN 

PROVIDER: S-EPMC5585990 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature