MicroRNAs participate in gene expression regulation and phytohormone cross-talk in barley embryo during seed development and germination.

ABSTRACT:

Background

Small RNA and degradome sequencing have identified a large number of miRNA-target pairs in plant seeds. However, detailed spatial and temporal studies of miRNA-mediated regulation, which can reflect links between seed development and germination are still lacking.

Results

In this study, we extended our investigation on miRNAs-involved gene regulation by a combined analysis of seed maturation and germination in barley. Through bioinformatics analysis of small RNA sequencing data, a total of 1324 known miRNA families and 448 novel miRNA candidates were identified. Of those, 16 known miRNAs with 40 target genes, and three novel miRNAs with four target genes were confirmed based on degradome sequencing data. Conserved miRNA families such as miR156, miR168, miR166, miR

SUBMITTER: Bai B 

PROVIDER: S-EPMC5586051 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature