Analysis of Large-Scale Mutagenesis Data To Assess the Impact of Single Amino Acid Substitutions.

ABSTRACT: Mutagenesis is a widely used method for identifying protein positions that are important for function or ligand binding. Advances in high-throughput DNA sequencing and mutagenesis techniques have enabled measurement of the effects of nearly all possible amino acid substitutions in many proteins. The resulting large-scale mutagenesis data sets offer a unique opportunity to draw general conclusions about the effects of different amino acid substitutions. Thus, we analyzed 34,373 mutations in 14 proteins whose effects were measured using large-scale mutagenesis approaches. Methionine was the most tolerated substitution, while proline was the least tolerated. We found that several substitutions, including histidine and asparagine, best recapitulated the effects of other substitutions, even whe

SUBMITTER: Gray VE 

PROVIDER: S-EPMC5586385 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature