Short-term spheroid culture of primary colorectal cancer cells as an in vitro model for personalizing cancer medicine.

ABSTRACT: Chemotherapy treatment of cancer remains a challenge due to the molecular and functional heterogeneity displayed by tumours originating from the same cell type. The pronounced heterogeneity makes it difficult for oncologists to devise an effective therapeutic strategy for the patient. One approach for increasing treatment efficacy is to test the chemosensitivity of cancer cells obtained from the patient's tumour. 3D culture represents a promising method for modelling patient tumours in vitro. The aim of this study was therefore to evaluate how closely short-term spheroid cultures of primary colorectal cancer cells resemble the original tumour. Colorectal cancer cells were isolated from human tumour tissue and cultured as spheroids. Spheroid cultures were established with a high success rat

SUBMITTER: Jeppesen M 

PROVIDER: S-EPMC5587104 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature