Escaping introns in COI through cDNA barcoding of mushrooms: Pleurotus as a test case.

ABSTRACT: DNA barcoding involves the use of one or more short, standardized DNA fragments for the rapid identification of species. A 648-bp segment near the 5' terminus of the mitochondrial cytochrome c oxidase subunit I (COI) gene has been adopted as the universal DNA barcode for members of the animal kingdom, but its utility in mushrooms is complicated by the frequent occurrence of large introns. As a consequence, ITS has been adopted as the standard DNA barcode marker for mushrooms despite several shortcomings. This study employed newly designed primers coupled with cDNA analysis to examine COI sequence diversity in six species of Pleurotus and compared these results with those for ITS. The ability of the COI gene to discriminate six species of Pleurotus, the commonly cultiva

SUBMITTER: Avin FA 

PROVIDER: S-EPMC5587487 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature