Discovery and characterization of single nucleotide polymorphisms in two anadromous alosine fishes of conservation concern.

ABSTRACT: Freshwater habitat alteration and marine fisheries can affect anadromous fish species, and populations fluctuating in size elicit conservation concern and coordinated management. We describe the development and characterization of two sets of 96 single nucleotide polymorphism (SNP) assays for two species of anadromous alosine fishes, alewife and blueback herring (collectively known as river herring), that are native to the Atlantic coast of North America. We used data from high-throughput DNA sequencing to discover SNPs and then developed molecular genetic assays for genotyping sets of 96 individual loci in each species. The two sets of assays were validated with multiple populations that encompass both the geographic range and the known regional genetic stocks of both species. The SNP pan

SUBMITTER: Baetscher DS 

PROVIDER: S-EPMC5587496 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature