Calcium ion regulation by BAPTA-AM and ruthenium red improved the fertilisation capacity and developmental ability of vitrified bovine oocytes.

ABSTRACT: Vitrification reduces the fertilisation capacity and developmental ability of mammalian oocytes; this effect is closely associated with an abnormal increase of cytoplasmic free calcium ions ([Ca²⁺]i). However, little information about the mechanism by which vitrification increases [Ca²⁺]i levels or a procedure to regulate [Ca²⁺]i levels in these oocytes is available. Vitrified bovine oocytes were used to analyse the effect of vitrification on [Ca²⁺]i, endoplasmic reticulum Ca²⁺ (ER Ca²⁺), and mitochondrial Ca²⁺ (mCa²⁺) levels. Our results showed that vitrification, especially with dimethyl sulfoxide (DMSO), can induce ER Ca²⁺ release into the cytoplasm, consequently increasing the [Ca^2+<

SUBMITTER: Wang N 

PROVIDER: S-EPMC5587528 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature