Tilt-less 3-D electron imaging and reconstruction of complex curvilinear structures.

ABSTRACT: The ability to obtain three-dimensional (3-D) information about morphologies of nanostructures elucidates many interesting properties of materials in both physical and biological sciences. Here we demonstrate a novel method in scanning transmission electron microscopy (STEM) that gives a fast and reliable assessment of the 3-D configuration of curvilinear nanostructures, all without needing to tilt the sample through an arc. Using one-dimensional crystalline defects known as dislocations as a prototypical example of a complex curvilinear object, we demonstrate their 3-D reconstruction two orders of magnitude faster than by standard tilt-arc TEM tomographic techniques, from data recorded by selecting different ray paths of the convergent STEM probe. Due to its speed and immunity to problem

SUBMITTER: Oveisi E 

PROVIDER: S-EPMC5587565 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature