Iron-dependent reconfiguration of the proteome underlies the intracellular lifestyle of Brucella abortus.

ABSTRACT: Brucella ssp. is a facultative intracellular pathogen that causes brucellosis, a worldwide zoonosis that affects a wide range of mammals including humans. A critical step for the establishment of a successful Brucella infection is its ability to survive within macrophages. To further understand the mechanisms that Brucella utilizes to adapt to an intracellular lifestyle, a differential proteomic study was performed for the identification of intracellular modulated proteins. Our results demonstrated that at 48 hours post-infection Brucella adjusts its metabolism in order to survive intracellularly by modulating central carbon metabolism. Remarkably, low iron concentration is likely the dominant trigger for reprogramming the protein expression profile. Up-regulation of proteins dedicated to

SUBMITTER: Roset MS 

PROVIDER: S-EPMC5587712 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature