Rate-limiting steps in transcription dictate sensitivity to variability in cellular components.

ABSTRACT: Cell-to-cell variability in cellular components generates cell-to-cell diversity in RNA and protein production dynamics. As these components are inherited, this should also cause lineage-to-lineage variability in these dynamics. We conjectured that these effects on transcription are promoter initiation kinetics dependent. To test this, first we used stochastic models to predict that variability in the numbers of molecules involved in upstream processes, such as the intake of inducers from the environment, acts only as a transient source of variability in RNA production numbers, while variability in the numbers of a molecular species controlling transcription of an active promoter acts as a constant source. Next, from single-cell, single-RNA level time-lapse microscopy of independent lineag

SUBMITTER: Makela J 

PROVIDER: S-EPMC5587725 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature