Towards designer organelles by subverting the peroxisomal import pathway.

ABSTRACT: The development of 'designer' organelles could be a key strategy to enable foreign pathways to be efficiently controlled within eukaryotic biotechnology. A fundamental component of any such system will be the implementation of a bespoke protein import pathway that can selectively deliver constituent proteins to the new compartment in the presence of existing endogenous trafficking systems. Here we show that the protein-protein interactions that control the peroxisomal protein import pathway can be manipulated to create a pair of interacting partners that still support protein import in moss cells, but are orthogonal to the naturally occurring pathways. In addition to providing a valuable experimental tool to give new insights into peroxisomal protein import, the variant receptor-signal seq

SUBMITTER: Cross LL 

PROVIDER: S-EPMC5587766 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature