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Enhancement of the productivity of the potent bacteriocin avicin A and improvement of its stability using nanotechnology approaches.

ABSTRACT: Herein, enhancements of the yield and antimicrobial activity duration of the bacteriocin avicin A were accomplished using fractional factorial design (FFD) and layered double hydroxide (LDH) nanoparticles. Firstly, potential factors affecting bacteriocin production were selected for preliminary study. By a 25-1 FFD, high pH was shown to have a positive effect on avicin A yield, while temperature and duration of incubation, as well as peptone nitrogen sources all had negative effects. The highest bacteriocin production and activity (2560?BU/ml) were observed after 30?h of incubation at 30?°C, with pH adjustment at 7, and in the presence of 2?g mannitol as carbon source and 2.2?g peptone as nitrogen source. Secondly, avicin A nanocomposites with different LDH precursors were tested. Only avicin A-ZnAl-CO3 LDH demonstrated a potent antimicrobial activity against Lactobacillus sakei LMGT 2313 that lasted for at least 24 days, as compared to the values of 6 and 15 days observed with the free avicin A that has been stored at room temperature and at 4?°C, respectively. In conclusion, avicin A production and stability can be improved by manipulating the growth conditions and media composition, together with conjugation to LDHs.

SUBMITTER: Fahim HA

PROVIDER: S-EPMC5587769 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Enhancement of the productivity of the potent bacteriocin avicin A and improvement of its stability using nanotechnology approaches.

Fahim Hazem A HA Rouby Waleed M A El WMAE El-Gendy Ahmed O AO Khairalla Ahmed S AS Naguib Ibrahim A IA Farghali Ahmed A AA

Scientific reports 20170906 1

Herein, enhancements of the yield and antimicrobial activity duration of the bacteriocin avicin A were accomplished using fractional factorial design (FFD) and layered double hydroxide (LDH) nanoparticles. Firstly, potential factors affecting bacteriocin production were selected for preliminary study. By a 2<sup>5-1</sup> FFD, high pH was shown to have a positive effect on avicin A yield, while temperature and duration of incubation, as well as peptone nitrogen sources all had negative effects. ...[more]

PMID: 28878272

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Enhancement of the productivity of the potent bacteriocin avicin A and improvement of its stability using nanotechnology approaches.

Publications

Enhancement of the productivity of the potent bacteriocin avicin A and improvement of its stability using nanotechnology approaches.

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