Temporal Regulation of a Salmonella Typhimurium Virulence Factor by the Transcriptional Regulator YdcR.

ABSTRACT: We previously examined Salmonella proteome within infected host cells and found differential expression of many proteins with defined functional roles such as metabolism or virulence. However, the precise roles of other altered proteins in Salmonella pathogenesis are largely unknown. A putative transcriptional regulator, YdcR, was highly induced intracellularly whereas barely expressed in vitro, implicating potential relevance to bacterial infection. To unveil its physiological functions, we exploited quantitative proteomics of intracellular Salmonella and found that genetic ablation of ydcR resulted in severe repression of SrfN, a known virulence factor. Immunoblotting, qRT-PCR, and β-galactosidase assays further demonstrate YdcR-dependent transcription

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5587866 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature