Data for automated, high-throughput microscopy analysis of intracellular bacterial colonies using spot detection.

ABSTRACT: Quantification of intracellular bacterial colonies is useful in strategies directed against bacterial attachment, subsequent cellular invasion and intracellular proliferation. An automated, high-throughput microscopy-method was established to quantify the number and size of intracellular bacterial colonies in infected host cells (Detection and quantification of intracellular bacterial colonies by automated, high-throughput microscopy, Ernstsen et al., 2017 [1]). The infected cells were imaged with a 10× objective and number of intracellular bacterial colonies, their size distribution and the number of cell nuclei were automatically quantified using a spot detection-tool. The spot detection-output was exported to Excel, where data analysis was performed. In this article, micrographs and spo

SUBMITTER: Ernstsen CL 

PROVIDER: S-EPMC5587884 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature