Determination of optimal sites of antisense oligonucleotide cleavage within TNFalpha mRNA.

ABSTRACT: Antisense oligonucleotides provide a powerful tool in order to determine the consequences of the reduced expression of a selected target gene and may include target validation and therapeutic applications. Methods of predicting optimum antisense sites are not always effective. We have compared the efficacy of antisense oligonucleotides, which were selected in vitro using random combinatorial oligonucleotide libraries of differing length and complexity, upon putative target sites within TNFalpha mRNA. The relationship of specific target site accessibility and oligonucleotide efficacy with respect to these parameters proved to be complex. Modification of the length of the recognition sequence of the oligonucleotide library illustrated that independent target sites demonstrated a preference f

SUBMITTER: Lloyd BH 

PROVIDER: S-EPMC55886 | biostudies-literature | 2001 Sep

REPOSITORIES: biostudies-literature