Extracellular matrix microarrays to study inductive signaling for endoderm specification.

ABSTRACT: During tissue development, stem and progenitor cells are faced with fate decisions coordinated by microenvironmental cues. Although insights have been gained from in vitro and in vivo studies, the role of the microenvironment remains poorly understood due to the inability to systematically explore combinations of stimuli at a large scale. To overcome such restrictions, we implemented an extracellular matrix (ECM) array platform that facilitates the study of 741 distinct combinations of 38 different ECM components in a systematic, unbiased and high-throughput manner. Using embryonic stem cells as a model system, we derived definitive endoderm progenitors and applied them to the array platform to study the influence of ECM, including the interactions of ECM with growth factor signaling, on t

SUBMITTER: Malta DFB 

PROVIDER: S-EPMC5588684 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature