High-throughput PCR assay design for targeted resequencing using primerXL.

ABSTRACT:

Background

Although the sequencing landscape is rapidly evolving and sequencing costs are continuously decreasing, whole genome sequencing is still too expensive for use on a routine basis. Targeted resequencing of only the regions of interest decreases both costs and the complexity of the downstream data-analysis. Various target enrichment strategies are available, but none of them obtain the degree of coverage uniformity, flexibility and specificity of PCR-based enrichment. On the other hand, the biggest limitation of target enrichment by PCR is the need to design large numbers of partially overlapping assays to cover the target.

Results

To overcome the aforementioned hurdles, we have developed primerXL, a state-of-the-art PCR primer design pipeline for targeted resequenci

SUBMITTER: Lefever S 

PROVIDER: S-EPMC5588703 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature