In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases.

ABSTRACT:

Background

P450 fatty acid decarboxylases represented by the unusual CYP152 peroxygenase family member OleT_JE have been receiving great attention recently since these P450 enzymes are able to catalyze the simple and direct production of 1-alkenes for potential applications in biofuels and biomaterials. To gain more mechanistic insights, broader substrate spectra, and improved decarboxylative activities, it is demanded to discover and investigate more P450 fatty acid decarboxylases.

Results

Here, we describe for the first time the expression, purification, and in vitro biochemical characterization of two new CYP152 peroxygenases, CYP-Aa162 and CYP-Sm46Δ29, that are capable of decarboxylating straight-chain saturated fatty acids. Both enzymes were found to catalyze

SUBMITTER: Xu H 

PROVIDER: S-EPMC5588734 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature