Full-length nucleotide sequence of ERMAP alleles encoding Scianna (SC) antigens.

ABSTRACT:

Background

Scianna (SC) blood group system comprises two antithetical antigens, Sc1 and Sc2, and five additional antigens. The antigens reside on a glycoprotein encoded by the erythroblast membrane-associated protein (ERMAP) gene. For the common ERMAP alleles, we determined the full-length nucleotide sequence that encodes the Scianna glycoprotein.

Study design and methods

Blood donor samples from five populations were analyzed including 20 African Americans, 10 Caucasians, 10 Thai, five Asians, and five Hispanics for a total of 100 chromosomes. An assay was devised to determine the genomic sequence of the ERMAP gene in one amplicon, spanning 21.4 kb and covering Exons 2 to 12 and the intervening sequence (IVS). All alleles (confirmed haplotypes) were resolved without ambigui

SUBMITTER: Srivastava K 

PROVIDER: S-EPMC5588880 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature