Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.

ABSTRACT: A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.

SUBMITTER: Robinson PJ 

PROVIDER: S-EPMC5589196 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature