Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells.

ABSTRACT: Replacement of lost beta cells in patients with diabetes has the potential to alleviate them of their disease, yet current protocols to make beta cells are inadequate for therapy. In vitro screens can reveal the signals necessary for endocrine maturation to improve beta cell production, however the complexities of in vivo development that lead to beta cell formation are lost in two-dimensional systems. Here, we create three-dimensional organotypic pancreatic cultures, named pancreatoids, composed of embryonic day 10.5 murine epithelial progenitors and native mesenchyme. These progenitors assemble in scaffold-free, floating conditions and, with the inclusion of native mesenchyme, develop into pancreatoids expressing markers of different pancreatic lineages including endocrine-like cells. Tr

SUBMITTER: Scavuzzo MA 

PROVIDER: S-EPMC5589819 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature