Monolithic Chip for High-throughput Blood Cell Depletion to Sort Rare Circulating Tumor Cells.

ABSTRACT: Circulating tumor cells (CTCs) are a treasure trove of information regarding the location, type and stage of cancer and are being pursued as both a diagnostic target and a means of guiding personalized treatment. Most isolation technologies utilize properties of the CTCs themselves such as surface antigens (e.g., epithelial cell adhesion molecule or EpCAM) or size to separate them from blood cell populations. We present an automated monolithic chip with 128 multiplexed deterministic lateral displacement devices containing ~1.5 million microfabricated features (12 µm-50 µm) used to first deplete red blood cells and platelets. The outputs from these devices are serially integrated with an inertial focusing system to line up all nucleated cells for multi-stage magnetophoresis to remove magnet

SUBMITTER: Fachin F 

PROVIDER: S-EPMC5589885 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature