Planar polarized Rab35 functions as an oscillatory ratchet during cell intercalation in the Drosophila epithelium.

ABSTRACT: The coordination between membrane trafficking and actomyosin networks is essential to the regulation of cell and tissue shape. Here, we examine Rab protein distributions during Drosophila epithelial tissue remodeling and show that Rab35 is dynamically planar polarized. Rab35 compartments are enriched at contractile interfaces of intercalating cells and provide the first evidence of interfacial monopolarity. When Rab35 function is disrupted, apical area oscillations still occur and contractile steps are observed. However, contractions are followed by reversals and interfaces fail to shorten, demonstrating that Rab35 functions as a ratchet ensuring unidirectional movement. Although actomyosin forces have been thought to drive interface contraction, initiation of Rab35 compartments does not r

SUBMITTER: Jewett CE 

PROVIDER: S-EPMC5589913 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature