Cancer-associated fibroblasts promote bone invasion in oral squamous cell carcinoma.

ABSTRACT:

Background

The molecular mechanisms involved in the invasion of bone by oral squamous cell carcinomas (OSCC) are poorly understood, and little is known about the role of cancer-associated fibroblasts (CAF), the presence of which confers a poor prognosis.

Methods

Clinicopathological data from 277 OSCC cases involving bone resections were reviewed, and 32 cases thoroughly analysed histologically. Immunohistochemistry was used to examine αSMA, RANKL and OPG. Western blotting and qPCR were used to assess myofibroblast (CAF-like) differentiation, RANKL and OPG expression in vitro, and RANKL secretion was analysed by ELISA. Osteoclastogenesis was examined using TRAP staining, multinucleation and pit forming assays.

Results

Fibrous stroma intervened between tumour and bone i

SUBMITTER: Elmusrati AA 

PROVIDER: S-EPMC5589989 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature