Human Cell Line-Derived Monoclonal IgA Antibodies for Cancer Immunotherapy.

ABSTRACT: IgA antibodies have great potential to improve the functional diversity of current IgG antibody-based cancer immunotherapy options. However, IgA production and purification is not well established, which can at least in part be attributed to the more complex glycosylation as compared to IgG antibodies. IgA antibodies possess up to five N-glycosylation sites within their constant region of the heavy chain as compared to one site for IgG antibodies. The human GlycoExpress expression system was developed to produce biotherapeutics with optimized glycosylation and used here to generate a panel of IgA isotype antibodies directed against targets for solid (TA-mucin 1, Her2, EGFR, Thomsen-Friedenreich) and hematological (CD20) cancer indications. The feasibility of good manufacturing pract

SUBMITTER: Hart F 

PROVIDER: S-EPMC5590476 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature