Domain-based prediction of the human isoform interactome provides insights into the functional impact of alternative splicing.

ABSTRACT: Alternative splicing is known to remodel protein-protein interaction networks ("interactomes"), yet large-scale determination of isoform-specific interactions remains challenging. We present a domain-based method to predict the isoform interactome from the reference interactome. First, we construct the domain-resolved reference interactome by mapping known domain-domain interactions onto experimentally-determined interactions between reference proteins. Then, we construct the isoform interactome by predicting that an isoform loses an interaction if it loses the domain mediating the interaction. Our prediction framework is of high-quality when assessed by experimental data. The predicted human isoform interactome reveals extensive network remodeling by alternative splicing. Protein pairs in

SUBMITTER: Ghadie MA 

PROVIDER: S-EPMC5591010 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature