Analysis of mutations in the SOS-1 gene in two Polish families with hereditary gingival fibromatosis.

ABSTRACT:

Objectives

To establish whether two families from Malopolska and Mazovia provinces in Poland are affected by hereditary gingival fibromatosis type 1, caused by a single-cytosine insertion in exon 21 of the Son-of-Sevenless-1 gene.

Material and methods

Six subjects with hereditary gingival fibromatosis and five healthy subjects were enrolled in the study. Gingival biopsies were collected during gingivectomy or tooth extraction and used for histopathological evaluation. Total RNA and genomic DNA were purified from cultured gingival fibroblasts followed by cDNA and genomic DNA sequencing and analysis.

Results

Hereditary gingival fibromatosis was confirmed by periodontal examination, X-ray, and laboratory tests. Histopathological evaluation showed hyperplastic epithelium,

SUBMITTER: Gawron K 

PROVIDER: S-EPMC5591049 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature