Sulfonolipids as novel metabolite markers of Alistipes and Odoribacter affected by high-fat diets.

ABSTRACT: The gut microbiota generates a huge pool of unknown metabolites, and their identification and characterization is a key challenge in metabolomics. However, there are still gaps on the studies of gut microbiota and their chemical structures. In this investigation, an unusual class of bacterial sulfonolipids (SLs) is detected in mouse cecum, which was originally found in environmental microbes. We have performed a detailed molecular level characterization of this class of lipids by combining high-resolution mass spectrometry and liquid chromatography analysis. Eighteen SLs that differ in their capnoid and fatty acid chain compositions were identified. The SL called "sulfobacin B" was isolated, characterized, and was significantly increased in mice fed with high-fat diets. To reveal bacterial

SUBMITTER: Walker A 

PROVIDER: S-EPMC5591296 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature