A Streamlined Approach to Antibody Novel Germline Allele Prediction and Validation.

ABSTRACT: Advancements in high-throughput sequencing and molecular identifier-based error correction have opened the door to antibody repertoire sequencing with single mutation precision, increasing both the breadth and depth of immune response characterization. However, improvements in sequencing technology cannot resolve one key aspect of antibody repertoire sequencing accuracy: the possibility of undocumented novel germline alleles. Somatic hypermutation (SHM) calling requires a reference germline sequence, and the antibody variable region gene alleles collected by the IMGT database, although large in number, are not comprehensive. Mismatches, resulted from single nucleotide polymorphisms or other genetic variation, between the true germline sequence and the closest IMGT allele can inflate SHM co

SUBMITTER: Wendel BS 

PROVIDER: S-EPMC5591497 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature